Pat Reddington (Valenzuela) using the TEM (Transmission Electron Microscope) at Penn. While Drexel has a TEM, finding time on either machine can be difficult, as these instruments are often rented out to corporations. The TEM works much like an optical microscope, with in this case and leectron beam "shining" through a very thin sample
We depend on this microscope, as well as the SEM The scanning electron microscope (SEM) is a type of electron microscope that images the sample surface by scanning it with a high-energy beam of electrons in a raster scan pattern. The electrons interact with the atoms that make up the sample producing signals that contain information about the sample's surface topography, composition and other properties such as electrical conductivity. Hoeever in this case the electrons reflect back from the sample. 
My initial reseach work involved reducing silcon particles to nano-particales about 5 nm in size. To do this requred hydrofluoric & nitric acid with diluents - Deionized water and methanol. HF is one of the more dangerous substances we work with in the lab, and using require a great deal of caution. The blue box contains calcium glutamate, a neutalizing ageent that will also enter the blood stream and prevent the HF from depleating the blood of calcium, and important element in muscle contraction, especially the heart.
Here I'm preparing a dilute soluction of 2 mL HF, 0.2 mL (hence the micro pipette) of nitric acid, 15.4 mL of DI water. If you look carefully you will see a dime sized filter the pH neutralizing solution must pass through, drop by drop until all 270 mL of the DI and methanol solution are in the flask..